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λ phage dna  (New England Biolabs)


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    Structured Review

    New England Biolabs λ phage dna
    λ Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phage+lambda+dna/Lambda+DNA/pm42820372-137-10-13
    Average 97 stars, based on 1840 article reviews
    λ phage dna - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Control:

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress.
    Article Snippet: .. CC-seq-v2: Internal CC-seq Control Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
    Article Snippet: .. Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Lambda DNA Preparation:

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress.
    Article Snippet: .. CC-seq-v2: Internal CC-seq Control Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Article Title: Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro
    Article Snippet: .. 12.5 μg HindIII-digested phage lambda DNA was dephoshorylated in 1x Cutsmart buffer (NEB) with 10 U Quick-CIP phosphatase (NEB) in a final volume of 30 μl for 1 h at 37°C. .. 12.5 μg HindIII-digested phage lambda DNA was dephoshorylated in 1x Cutsmart buffer (NEB) with 10 U Quick-CIP phosphatase (NEB) in a final volume of 30 μl for 1 h at 37°C.

    Article Title: Nuclease-NTPase systems use shared molecular features to control bacterial anti-phage defense
    Article Snippet: BL21(DE3) gDNA was isolated using a DNeasy Blood and Tissue Kit (Qiagen). .. M13mp18 single-stranded DNA and phage Lambda DNA were purchased from NEB. ..

    Article Title: Nuclease-NTPase antiphage defence systems use conserved molecular features to control bacterial immunity.
    Article Snippet: Bacteria encode diverse defence systems, including restriction– modification and CRISPR–Cas, that cleave nucleic acid to protect against phage infection.. Bioinformatic analyses demonstrate that many recently identified antiphage defence operons comprise a nuclease and NTPase protein, suggesting that additional nucleic acid-targeting systems remain to be understood.. Here we develop large-scale comparative cell biology and biochemical approaches to analyse 16 nuclease–NTPase systems and define molecular features that control antiphage defence.

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
    Article Snippet: .. This was combined with phage lambda DNA (NEB) at a ratio of 1:50 (human DNA:lambda DNA W:W). ..

    Article Title: A simple and general approach to generate photoactivatable DNA processing enzymes
    Article Snippet: The samples were run in 10–12% urea–PAGE gels and the fluorescence signal was read in a Fusion FX6 EDGE imaging system (Vilber). .. The activity of the restriction endonuclease StuI was measured in both phage lambda DNA (New England Biolabs, USA) and a quencher-fluorophore dsDNA probe. .. The samples were run in 10–12% urea–PAGE gels and the fluorescence signal was read in a Fusion FX6 EDGE imaging system (Vilber).

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
    Article Snippet: .. Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Sonication:

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress.
    Article Snippet: .. CC-seq-v2: Internal CC-seq Control Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Article Title: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
    Article Snippet: .. Sheared etoposide (VP16)-treated human DNA plus sheared phage lambda DNA (NEB) was spiked in following the sonication stage of the protocol to permit internal quality control and potential calibration. ..

    Activity Assay:

    Article Title: A simple and general approach to generate photoactivatable DNA processing enzymes
    Article Snippet: The samples were run in 10–12% urea–PAGE gels and the fluorescence signal was read in a Fusion FX6 EDGE imaging system (Vilber). .. The activity of the restriction endonuclease StuI was measured in both phage lambda DNA (New England Biolabs, USA) and a quencher-fluorophore dsDNA probe. .. The samples were run in 10–12% urea–PAGE gels and the fluorescence signal was read in a Fusion FX6 EDGE imaging system (Vilber).



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